Allopregnanolone is made from progesterone which is converted into 5α-dihydroprogesterone by 5α-reductase type I, and 3α-hydroxysteroid oxidoreductase isoenzymes. This 5α-dihydro intermediate is then converted into allopregnanolone. 3α-hydroxysteroids do not interact with classical intracellular steroid receptors but bind stereoselectively and with high affinity to receptors for the major inhibitory neurotransmitter in brain, γ-amino-butyric acid (GABA). In addition, allopregnanolone has been shown to act as an agonist of membrane progesterone receptors (mPR) with activity approximately an order of magnitude higher than at the GABAA receptor.
Allopregnanolone may be involved in neuronal plasticity, learning and memory processes, aggression, depression, tremors and epilepsy, and the modulation of responses to stress, anxiety and depression. It aids neurogenesis and has been found to reverse neuron proliferative deficit and cognitive deficits in mouse models of Alzheimer’s disease. Allopregnanolone has also been shown to restore functionality in a mouse model of Parkinson’s Disease and to improve behavioral problems in post-traumatic stress disorder.
Our new DetectX® Allopregnanolone Monoclonal Antibody Based EIA kit (K061-H) is designed to quantitatively measure Allopregnanolone present in extracted serum, plasma, or dried fecal samples, or in diluted urine and tissue culture media samples. In addition to the reliability and long term supply stability offered by monoclonal antibodies, this new kit (K061-H) is more sensitive than our original allopregnanolone EIA kit (K044-H), provides increased signal, and has a better cross reactivity profile.
| Allopregnanolone Monoclonal Antibody Based EIA Kits | Allopregnanolone EIA Kit | Allopregnanolone Chemiluminescent Immunoassay Kit | |
| Catalog Number | K061-H | K044-H | K044-C |
| Sample Types | Extracted serum, EDTA or heparin plasma, dried fecal samples, diluted urine and tissue culture media | Extracted serum, EDTA or heparin plasma, dried fecal samples, diluted urine and tissue culture media | Extracted serum, EDTA or heparin plasma, dried fecal samples, diluted urine and tissue culture media |
| Sample Volume | 50 ul | 50 ul | 100 ul |
| Samples/Kit | 39 or 231 in duplicate | 38 or 230 in duplicate | 39 or 231 in duplicate |
| Standard Range | 10,000 – 156 pg/ml | 50,000 – 391 pg/ml | 5000 – 78 pg/ml |
| Time to Answer | 2.5 hours | 2.5 hours | Overnight (~17 hours) |
| Detection | Colorimetric | Colorimetric | Luminescence |
| Antibody type | monoclonal | polyclonal | polyclonal |
| Antibody Supply | unlimited | limited | limited |
| B0 Net OD signal | 0.954 | 0.491 | N/A |
| Sensitivity | 127 pg/ml | 130 pg/ml | 20.9 pg/ml |
| Limit of Detection | 65.9 pg/ml | 240 pg/ml | 50.6 pg/ml |
| Cross reactivity (top 3 by %) | THDOC 3.08%
Pregnanolone 2.19% Progesterone 0.12% |
Pregnanolone 2.26%
THDOC 1% DHDOC 0.38% |
Pregnanolone 2.26%
THDOC 1% DHDOC 0.38% |
For the time being the new Monoclonal Antibody-Based Allopregnanolone kit will be offered in parallel with the original polyclonal antibody based kit. Ultimately we believe that the new kit offers improvements for the vast majority of researchers, but you do not need to take our word for it. To encourage all customers to try the new kit for themselves we are offering an initial 1-Plate kit at a 50% discount.
Reference coupon code ALLO16E to receive a 50% discount on your first purchase of K061-H1. Offer is limited to one per customer, and is available to US customers only.
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