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- Sample Types Urine
- Species Human, Rat, Dog, Monkey
- Samples/Kit 88 or 472 in Duplicate
- Stability Liquid 4°C Stable Reagents
- Calibrated N-Cal Kit, NIST Standard Reference #914a
- Time to Answer 30 Minutes
- Format 96-Well
- Readout Colorimetric, 450 nm
- Standard Curve
Creatinine (2-amino-1-methyl-5H-imidazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally, altered creatinine levels may be associated with conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease.