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- Assay Type Detection Kit
- Sample Types Urine
- Sensitivity 0.019 mg/dL
- Species Species Independent
- Assay Duration 30 Minutes
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 490 nm
- Standard Curve
The Urinary Creatinine Detection Kit quantitatively measures Creatinine levels in urine. The Urinary Creatinine Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.
Use our provided Creatinine standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a clear microtiter plate. Add DetectX® Creatinine Reagent to each well tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at room temperature for 30 minutes. The color-generating reaction occurs between the DetectX® Creatinine Reagent and the Creatinine within the sample or standard.
After the 30-minute incubation, use a plate reader to detect and record the generated signal at 490nm. Use the intensity and the standard curve to calculate the Creatinine concentration in the samples.
Creatinine (2-amino-1-methyl-5H-imidazole-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that tissues can utilize for the production of ATP. We accumulate dietary Creatine or enzymatically synthesize Creatine from the amino acids arginine, glycine, and methionine. Synthesis mainly occurs in the kidneys and liver.
Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine. Creatinine diffuses into the blood with excretion by the kidneys. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a relatively constant rate. As intra-individual variation is <15% daily, Creatinine is a useful tool for normalizing the levels of other molecules found in urine. Altered creatinine levels may be associated with diabetes and cardiovascular disease.