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- Assay Type Detection Kit
- Sample Types Urine
- Sensitivity 0.019 mg/dL
- Species Human, Rat, Dog, Monkey
- Assay Duration 30 Minutes
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 490 nm
- Standard Curve
Assay Principle: The DetectX® Urinary Creatinine Detection Kit is designed to quantitatively measure creatinine present in urine samples of Human, Rat, Dog, and Monkey. The Urinary Creatine Detection Kit can detect Creatinine in as little as 30 minutes. Please read the complete kit insert before performing this assay.
Background: Creatinine (2-amino-1-methyl-5H-imidazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. Synthesis mainly occurs in the kidneys and liver.
Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine. Creatinine diffuses into the blood and is excreted by the kidneys. Creatinine forms spontaneously from p-creatine. Under normal conditions, its formation occurs at a rate that is relatively constant and as intra-individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Altered creatinine levels may be associated with conditions such as diabetes and cardiovascular disease.