20-Hydroxyecdysone ELISA Kit

Assay Principle: 

The DetectX® 20-Hydroxyecdysone ELISA Kit quantitatively measures 20-Hydroxyecdysone in tissue extracts. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided 20-Hydroxyecdysone standard to generate a standard curve for the assay.

Protocol Summary:

• Introduce standards or diluted samples into the provided transparent microtiter plate, pre-coated with goat anti-rabbit IgG antibody.
• Add 20-Hydroxyecdysone peroxidase conjugate and 20-Hydroxyecdysone rabbit polyclonal antibody to initiate the immunological reaction.
• Incubate the mixture covered at room temperature with shaking for 2 hours. The reaction varies inversely with the 20-Hydroxyecdysone concentration in the sample.
• After incubation, remove excess conjugate and add the TMB substrate, which reacts with the bound conjugate, producing a measurable signal.
• Use a plate reader to detect the signal at 450nm and calculate the 20-Hydroxyecdysone concentration based on the standard curve.

Background:

20-Hydroxyecdysone is a derivative of the insect molting hormone Ecdysone, first isolated in 1954. This steroid hormone regulates metamorphosis, cell death, and reproduction in arthropods. It is also found in a wide range of plant species, where it contributes to defense mechanisms against insect predation. The hormone’s fluctuating levels during metamorphosis activate signaling through the ecdysone receptor, crucial for transforming larvae and/or chrysalises into adult insects.

Recent studies have revealed the potential of 20-hydroxyecdysone in human medicine, particularly in enhancing osteogenesis and increasing bone mass. It has been observed to reduce cartilage degradation and boost protein synthesis, making it an area of interest for osteoporosis treatment and bodybuilding supplements aimed at muscle mass enhancement.

The DetectX® 20-Hydroxyecdysone ELISA Kit is a tool for researchers in entomology, phytochemistry, and medical research. It offers a reliable and sensitive method for quantifying 20-Hydroxyecdysone levels in various biological samples, aiding in the study of its diverse roles in both the animal and plant kingdoms.